Pramac Ducati rookie Francesco Bagnaia moved to the top of the times in second practice for this weekend's Italian Grand Prix at Mugello, ahead of fellow MotoGP newcomer Fabio Quartararo.
Bagnaia's best time of 1m46.732s came in the dying seconds of the Friday afternoon session as most riders opted to close out the day with a new soft rear tyre run.
Honda's Marc Marquez was one of the riders who opted against a new soft rear tyre and surpassed his own FP1 benchmark on only his second flying lap, setting a 1m47.062s that went unchallenged until the closing stages.
With six minutes left on the clock, Danilo Petrucci deposed the Honda rider by setting a 1m47.012s, before KTM's Pol Espargaro went fastest and became the first to break into the 1m46s range.
Petrucci responded with a 1m46.863s moments later, but that was then beaten by Quartararo's 1m46.778s on the Petronas Yamaha and then Bagnaia's last-ditch session-topping effort.
That meant Petrucci ended up third ahead of Espargaro, while Maverick Vinales was the fifth and final rider to crack the 1m47s barrier on the best of the works Yamahas.
Marquez's early lap was still good enough for sixth, followed by Alex Rins' Suzuki, Cal Crutchlow's LCR Honda and Jack Miller's Pramac Ducati.
Franco Morbidelli completed the top 10 and the provisional Q2 positions on the second Petronas SRT bike, fractionally ahead of Andrea Dovizioso's Ducati in 11th.
Johann Zarco was 12th on his KTM despite becoming the first to crash this weekend with a late fall at the sweeping Palagio left-hander.
That was soon followed by Miller running into the gravel and crashing at the following bend, Correntaio.
Aleix Espargaro once again led Aprilia's efforts, albeit down in 16th after ending up fifth in FP1.
Valentino Rossi was nearly a full second off the pace in 18th place.
Jorge Lorenzo was a lowly 20th on the second works Honda, 1.382s slower than Bagnaia.
Practice two times
|1||Francesco Bagnaia||Pramac Ducati||1m46.732s||-||16|
|2||Fabio Quartararo||Petronas Yamaha||1m46.778s||0.046s||18|
|8||Cal Crutchlow||LCR Honda||1m47.170s||0.438s||17|
|9||Jack Miller||Pramac Ducati||1m47.185s||0.453s||15|
|10||Franco Morbidelli||Petronas Yamaha||1m47.268s||0.536s||19|
|14||Takaaki Nakagami||LCR Honda||1m47.483s||0.751s||19|
|17||Tito Rabat||Avintia Ducati||1m47.686s||0.954s||18|
|21||Karel Abraham||Avintia Ducati||1m48.146s||1.414s||15|
|22||Hafizh Syahrin||Tech3 KTM||1m48.496s||1.764s||16|
|23||Miguel Oliveira||Tech3 KTM||1m48.914s||2.182s||20|