Joe Gibbs Racing NASCAR driver Kyle Busch will contest the Daytona 24 Hours for the first time in January 2020, driving an AIM Vasser-Sullivan Lexus GT3 car.
Busch, the 2015 NASCAR Cup Series champion, is the most successful Toyota NASCAR driver in its history of competing in the series, with 186 wins across the three major NASCAR championships.
Busch will race the RC F GT3 car - built by Toyota's sister brand Lexus - in the blue riband American GT race.
"I'd like to thank everyone at Lexus Motorsports, AIM Vasser Sullivan and Toyota for this opportunity," said Busch.
"To have the chance to run in such an iconic race as the Rolex 24 is certainly something I've thought about and wanted to do.
"My partnership with Toyota and the history we've had together has been incredible.
"I would love to continue that history and maybe get my own Daytona Rolex to add to my trophy collection."
Team co-owner and 1996 IndyCar champion Jimmy Vasser added: "We're thrilled to have a racer of Kyle's caliber join AIM Vasser Sullivan to open our 2020 season at the Rolex 24 at Daytona.
"Kyle has proven he can compete and win in many forms of motorsports and we look forward to having him drive the Lexus RC F GT3 at Daytona."
Busch holds the NASCAR record for the most wins in both the Xfinity Series (96) and the Truck Series (56) and is currently in the hunt for his second Cup title with two races remaining in the 2019 season.
He used Chevrolet power after making his NASCAR Cup debut with Hendrick Motorsports in 2005, but after switching to JGR for 2008, he has raced Toyota-powered cars in all of NASCAR's series.
"Kyle has been a fixture in our Toyota family for over a decade and is the winningest driver in the Toyota NASCAR programme," said Lisa Materazzo, vice president of marketing, Lexus Division.
"He has accomplished so many firsts for Toyota throughout his tenure and we couldn't be happier to have him join AIM Vasser Sullivan and drive the Lexus RC F GT3 in a world class event like the Rolex 24 at Daytona."
Busch will take part in the 2020 Roar - the event's official test - on January 3-5, before the Daytona 24 Hours takes place on January 25-26.