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Iannone passes Dovizioso at Turn 4 and sets off after Rossi.
Lorenzo's lead is up to 0.5s over Marquez at the head of the race.
Pedrosa, despite his ankle injury, is pressurising Smith for sixth place - the Spaniard has climbed up from ninth on the grid.
Here's the top five after six laps at Brno:
1 Lorenzo
2 Marquez
3 Rossi
4 Dovizioso
5 Iannone
1 Lorenzo
2 Marquez
3 Rossi
4 Dovizioso
5 Iannone
Rossi is now 4.4s behind the two leaders and he can't shake the Ducati pair, Dovizioso is just 0.5s behind the championship leader.
Replays show Eugene Laverty slid off at Turn 2 and he has retired from the race.
Pedrosa moves past Pol Espargaro to take seventh place.
Rossi is not catching Lorenzo and Marquez, he's now 3.6s behind the battle for the lead.
Marquez has a slight look at Lorenzo at the entrance to Turn 13 but Lorenzo shuts the door.
Stopwatch
Marquez is the fastest man on the track with a 1m56.158s last time through.
Rossi is 2.8s behind Marquez as the pack starts lap four.
Lorenzo leads Marquez by just 0.3s on lap three.
Rossi dives down the inside of Dovizioso at Turn 13 and slams the door shut at Turn 14 - the championship leader holds the position down the pit straight and he sets about chasing the leaders.
Lorenzo and Marquez are now 1.8s clear of the battle for third place.
Rossi leaps up the inside of Dovizioso at Turn 4, but the Ducati rider goes back in front on the exit of Turn five.
At the start of lap two, Lorenzo and Marquez are a second clear of Divizioso - Rossi is losing contact with his title rivals.
Dovizioso and Rossi have got passed Smith as Lorenzo and Marquez race clear at the front.
Bradley Smith got a great start and is up to third - Rossi is down to fifth.
Lorenzo leaps off the line and holds the line into Turn one.
Green flag
The lights are out and the Czech MotoGP is underway!
Lorenzo is the last non-Honda rider to win at Brno, a record that currently stretches back to 2010.
As the sun blazes down on the circuit, it's currently 27 degrees air temperature in Brno, but 40 degrees on the track.
The riders are off on their formation lap.
We're just five minutes away from the start of the Czech MotoGP and the grid is starting to clear.
Brno was also the setting for Rossi's first ever motorcycle victory on the Grand Prix scene - he won the 125cc class 19 years ago back in 1996.
Rossi is looking for a 13th consecutive podium finish - a record that stretches back to 2014 Japanese MotoGP at Motegi - and he hasn't finished lower than third place so far this season.
But after 10 races so far this season, it's Valentino Rossi who leads the MotoGP points standings with 195 ahead of the race at Brno. But his Yamaha teammate Jorge Lorenzo is just nine points behind in second place on 186.
Marquez is looking for his third win in succession after his wins at the Sachsenring and Indianapolis. The Spaniard currently sits third in the championship standings on 139 points.
Although he was visibly limping off the bike, Pedrosa was able to take part in qualifying and the Spaniard will line up in ninth place on the grid.
Lorenzo also topped FP1 and FP3, while Marquez topped a crashed filled FP2. Marquez's Dani Pedrosa, who won the Brno race in 2014, fell heavily in that session and injured his ankle.


We're 15 minutes away from the start of the Czech MotoGP race at Brno. Jorge Lorenzo is the man to beat after he broke the lap record twice on his way to pole position. Reigning world champion Marc Marquez lines up second and current championship leader Valentino Rossi will start third.
Lorenzo takes pole for Yamaha with new lap record

Lorenzo takes pole for Yamaha with new lap record

Zarco now leads Rabat by 79 points in the Moto2 standings, with Rins a further five points back in third. The Frenchman is on the top step of the podium for the fourth time this season. Next up in 35 minutes: the Czech MotoGP.
Quote
Zarco: "I think the control was good but when I had about sixth tenths advantage it was harder to get more.
"But I had really good pace with less fuel and the gap went out to seven, [then] eight tenths.
"It's great for the championship and now I have few days off before Silverstone."
"But I had really good pace with less fuel and the gap went out to seven, [then] eight tenths.
"It's great for the championship and now I have few days off before Silverstone."
Quote
Rabat: "Unfortunately I had a moment where I nearly crashed and Yohan had a little gap. It's a shame because I could have passed him. I pushed 100 per cent all the race."
Zarco does a back flip from a tyre barrier over his bike in celebration - just like in the race, he was in total control of the landing.
Here's the top ten finishing order of the Moto2 race at Brno:
1 Zarco
2 Rabat
3 Rins
4 Marquez
5 Lowes
6 Folger
7 Luthi
8 Cortese
9 Salom
10 Morbidelli
1 Zarco
2 Rabat
3 Rins
4 Marquez
5 Lowes
6 Folger
7 Luthi
8 Cortese
9 Salom
10 Morbidelli
That's Rabat's fourth win of the season and this will be his tenth visit to the Moto2 podium in succession.
Checkered flag
Zarco wins the Moto2 race at Brno!
Rins is within 0.5s of Rabat - but it looks like its to late for him to steal second.
Folger has closed to within 0.3s of Lowes in the scrap for fifth place.
By: Mitchell Adam, Alex Kalinauckas
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