Gigawave Motorsport has withdrawn its Aston Martin from this year's Le Mans 24 Hours.
The Essex-based squad, which secured three third places in the FIA GT Series last year, had planned to run its Aston DBR9 in the LM GT1 class at Le Mans in June.
The announcement of its withdrawal came shortly after it signed a technical agreement with NISMO to homologate the new Nissan GTR and develop the car's race programme in the FIA GT series for 2010.
Gigawave's chairman Henry Barcynski said: "It was an honour for Gigawave Motorsport to have been given the opportunity to compete in this year's Le Mans 24 Hour race."
"The 24 Hour race and the Le Mans Series have always been the ultimate challenge for the team, but watch this space because we will be back."
The first reserve on the entry list - Seattle Advanced Engineering - will fill the gap left by Gigawave.
The team will run a Ferrari F430 GT in the GT2 class with actor Patrick Dempsey (famous for his role in Grey's Anatomy) confirmed as part of the driver line-up.