Laguna Seca has lost its place on the 2014 MotoGP calendar.
The circuit first hosted the series in 1988, and has been on the schedule every year since 2005.
This year it was one of three US tracks on the MotoGP schedule, along with Indianapolis and the new Austin circuit at Texas.
A statement from the circuit said that trio of races had proved unsustainable however, and that Laguna Seca could not compete with the other venues.
"For 25 years, Laguna Seca has served as the 'home' for grand prix motorcycle racing in the United States, having staged 15 world championship grands prix since 1988," the statement read.
"Unfortunately, and in spite of loyal support from fans, sponsors and media, there will not be a 16th US Grand Prix in 2014.
"At this time, the US is only able to support two MotoGP events.
"The support provided by the states of Texas and Indiana make it difficult for us, as a not-for-profit, to currently compete.
"Laguna Seca extends its gratitude to all those whose combined efforts made the US Grand Prix such an iconic event; it took all of you - fans, sponsors, motorcycle community, media, volunteers and, not the least, Dorna Sports.
"Our pledge to you is to work diligently to return the MotoGP World Championship to Monterey, Calif., in the very near future."